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OcuLife ™ Epithelial Medium Complete Kit LL-0032

更新日期:2014年03月21日 浏览次数:
名称:OcuLife ™ Epithelial Medium Complete Kit LL-0032

货号:LL-0032

英文名称:OcuLife™ Medium Complete Kit

       (OcuLife Basal Medium, OcuLife LifeFactors® Kit)

价格:1879.00RMB

产品说明书:点击下载
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OcuLifeTM cell culture medium is optimized for the culture of Human Corneal Epithelial Cells.

OcuLifeTM supports the growth of these cells over a period of at least three passages.

OcuLifeTM  Basal Medium contains no antimicrobials and no phenol red. Lifeline® offers antimicrobials and phenol red, but they are not required for eukaryotic cell proliferation.

  • Kit format for formulation flexibility: Offered in a kit format composed of basal medium and associated supplements and growth factors called LifeFactors®. This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
  • Protective packaging: OcuLifeTM Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors®(growth factors and supplements) are packaged in a convenient gas-impermeable pouch for easy storage.

Corneal Epithelial CellsOcuLifeTM Medium grows Human Corneal Epithelial Cells with optimal morphology over a period of at least three passages. In comparisons with other commercially available media, OcuLifeTM shows superior proliferation at different seeding densities.

Shown left: Human Corneal Epithelial Cells, passage 3, 8 days after inoculation at 5,000 cells/cm2 (100X).

Shown below: Human Corneal Epithelial Cells inoculated at the stated density in 24-well plates and stained with crystal violet. Higher O.D. 590 represents a greater number of cells per well and therefore, better proliferation.

Corneal Epithelial Medium Comparison

LifeFactors® include:

OcuLife™ Medium is tested for:

  • Sterility:  Negative for bacteria and fungal growth
  • pH:  7.5 +/- 0.2
  • Cell Testing: Rate of proliferation and morphology
  • Osmolality:  315 +/- 10 mOsm
  • Endotoxin:  < 0.5 EU/mL

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